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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Leveraging and manufacturing in vitro multicellular spheroid-based tumor cell model as a preclinical tool for translating dysregulated tumor metabolism into clinical targets and biomarkers

Fig. 3

Adaptable bioreactor culture system can be used for massive production of multicellular tumor spheroids serving to preclinical drug screening and evaluation. Process sensors can aid the real-time observation and regulation of process parameters, and the microenvironment surrounding the MTSs can be manipulated to allow the environment (e.g., nutrients, metabolic wastes and cell types) in the bioreactor can be as close as possible to the microenvironment of tumor in vivo. To ensure that the MTSs generated in the bioreactor can be readily applied to preclinical biomedical research, comparing phenotypic landscapes between these individualized MTSs and solid tumors in patients is a must; the resulting MTSs similar to human solid tumors could be used in drug screening, gene therapy and tumor metabolomics research

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