Production of biodegradable microbial polymers from whey

Zikmanis, Peteris; Kolesovs, Sergejs; Semjonovs, Pavels

doi:10.1186/s40643-020-00326-6

Bioresources and Bioprocessing

Table 2 Comparatively efficient microbial production of polyhydroxyalkanoates (PHAs) from whey and whey-derived products ^a)

From: Production of biodegradable microbial polymers from whey

Substrate	Type of cultures/producing strain	Culture method	Type of PHA	Volumetric productivity, g/L/h	References
Lactose (Equimolar mixtures of glucose and galactose)	Pure culture/Pseudomonas cepacia ATCC 17759	Shaking flask	PHB	0.18	Young et al. 1994
Lactose whey supernatant	Pure culture/Methylobacterium sp. ZP24	Shaking flask, Bioreactor, Fed-Batch	PHB	0.08 0.09	Yellore and Desai 1998; Nath et al. 2008
Dairy waste	Pure culture/Bacillus megaterium SRKP-3	Bioreactor, Fed-Batch	PHB	0.31	Ram Kumar Pandian et al. 2010
Enzymatically hydrolysed whey permeate + 3HV^b and 4HB^c precursors	Pure culture/Haloferax mediterranei DSM 1411	Bioreactor, Fed-Batch	P-(3HB-co-3HV-co-4HB)	0.14	Koller et al. 2007, Koller et al. 2012
Whey powder supernatant + additives	Pure culture/Alcaligenes latus ATCC 29714	Shaking flask, Bioreactor, Batch	PHB	0.11	Berwig et al. 2016
Hydrolysed whey permeate + additives	Pure culture/Cupriavidus necator (Ralstonia eutropha DSM 545)	Bioreactor, Fed-Batch	P-(3HB-co-3HV)	0.17	Marangoni et al. 2002
Hydrolysed whey powder supernatant + additives	Pure culture/Haloferax mediterranei ATCC 33500	Bioreactor, Batch	P-(3HB-co-3HV)	0.16	Pais et al. 2016
Fermented whey powder permeate	MMC ^d/Undefined	Bioreactor, Fed-Batch	P-3(HB-co-HV)	0.56	Duque et al. 2014
Fermented whey supernatant	MMC/Undefined	Bioreactor, Fed-Batch	PHB/P-3(HB-co-HV)	0.45	Colombo et al. 2016
Processed whey powder solution	Engineered culture/rec. Escherichia coli CGSC 4401, harbouring pJC4 (Alcaligenes latus PHA biosynthesis genes)	Bioreactor, Fed-Batch	PHB	1.42 2.57 4.60 1.35	Ahn et al. 2000a, Ahn et al. 2001, Park et al. 2002
Hydrolysed whey permeate + additives	Engineered culture/rec. Cupriavidus necator mRePT (E. coli lactose degradation genes)	Bioreactor, Batch	PHB	0.70	Povolo et al. 2010

^aData summarised in recent specialised reviews (Amaro et al. 2019; Koller, 2016)
^b,^cP3-Hydroxyvalerate (HV) and 4-hydroxybutyrate (HB), precursors pentanoic acid and γ-butyrlactone, respectively
^dMixed microbial cultures, including consortia of activated sludge to produce volatile fatty acids, the precursors for PHAs’ biosynthesis

Back to article page