Table 1 Walne medium composition (Anderson 2005; Oo et al. 2017)

I

A Solution (per liter distilled water)

FeCl₃. 6H₂O

1.3 g

MnCl₂. 4H₂O

0.36 g

H₃BO₃

33.6 g

EDTA

45 g

NaH₂PO₄. 2H₂O

20 g

NaNO₃

100 g

B Solution

1 mL

II

B Solution (per 100 mL distilled water)

ZnCl₂

2.1 g

CoCl₂. 6H2O

2 g

(NH₄)₆Mo₇O₂₄. 4H₂O

0.9 g

CuSO₄. 5H₂O

2 g

Concentrated HCl

10 mL

III

C Solution (per 200 mL distilled water)

Vitamin B1

0.2 g

Vitamin B12

10 mg

Medium

1.

A Solution

1 mL/L

2.

B Solution

0.1 mL/L

3.

Sterile seawater

1 L