Skip to main content

Table 1 Walne medium composition (Anderson 2005; Oo et al. 2017)

From: Enhanced biomass productivity of microalgae Nannochloropsis sp. in an airlift photobioreactor using low-frequency flashing light with blue LED

Stock No. Substances Volume
I A Solution (per liter distilled water)
  1. FeCl3. 6H2O 1.3 g
  2. MnCl2. 4H2O 0.36 g
  3. H3BO3 33.6 g
  4. EDTA 45 g
  5. NaH2PO4. 2H2O 20 g
  6. NaNO3 100 g
  7. B Solution 1 mL
II B Solution (per 100 mL distilled water)
  1. ZnCl2 2.1 g
  2. CoCl2. 6H2O 2 g
  3. (NH4)6Mo7O24. 4H2O 0.9 g
  4. CuSO4. 5H2O 2 g
  5. Concentrated HCl 10 mL
III C Solution (per 200 mL distilled water)
  1. Vitamin B1 0.2 g
  2. Vitamin B12 10 mg
Medium
1. A Solution 1 mL/L
2. B Solution 0.1 mL/L
3. Sterile seawater 1 L