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Table 1 Thermodynamic parameters for the reverse hydrolysis from glucose to laminaribiose, cellobiose, and sophorose

From: A novel strategy for efficient disaccharides synthesis from glucose by β-glucosidase

Process

Keq (× 10−3)

rG0 (kJ/mol)

rG’ (kJ/mol)

2 Glucose = laminaribiose + H2O

3.5

14.0 ± 6.0

− 44.7 ± 6.0

2 Glucose = cellobiose + H2O

5.4

12.9 ± 3.7

-45.8 ± 3.7

2 Glucose = sophorose + H2O

3.4

14.1 ± 5.0

− 44.6 ± 5.0

  1. rG0: the change in Gibbs free energy of the chemical reaction in standard 1 M concentrations of substrates and products at pH 7.4 with ionic strength of 0.05 M
  2. Keq: the equilibrium constant of the chemical reaction in standard 1 M concentrations of substrates and products at pH 7.4 with ionic strength of 0.05 M
  3. rG’: the change in Gibbs free energy of the chemical reaction in 4.4 M concentrations of substrate at pH 7.4 with ionic strength of 0.05 M