Table 5 Biomass, lysine consumption, DAP titer, DAP productivity, yield of in vivo time-course with pSU-T7-CadA plasmid and 50 g/L lysine in BL21(DE3), W3110::IL5, W3110::L5 and W3110::pI, respectively
Strain | Time (h) | Lysine consumption (g/L) | DAP titer (g/L) | Productivity (g-DAP/L/h) | Yield (g/g, %) |
|---|---|---|---|---|---|
BL21(DE3) (+) | 6 | 24.21 ± 1.3 | 18.3 ± 1.2 | 3.05 ± 0.05 | 51.3 ± 1.2 |
12 | 38.94 ± 2.2 | 32.4 ± 2.5 | 2.70 ± 0.02 | 90.7 ± 3.2 | |
24 | 41.59 ± 1.9 | 28.0 ± 1.5 | 1.17 ± 0.01 | 78.4 ± 4.3 | |
W3110::IL5 (+) | 6 | 16.56 ± 0.6 | 12.8 ± 0.5 | 2.13 ± 0.03 | 35.9 ± 2.9 |
12 | 31.14 ± 1.1 | 22.4 ± 1.1 | 2.60 ± 0.04 | 62.7 ± 1.6 | |
24 | 37.35 ± 1.6 | 23.9 ± 0.9 | 1.00 ± 0.01 | 66.9 ± 1.8 | |
W3110::L5 (+) | 6 | 19.92 ± 1.0 | 15.4 ± 0.3 | 2.56 ± 0.02 | 43.1 ± 1.9 |
12 | 37.50 ± 2.4 | 29.3 ± 0.5 | 2.44 ± 0.05 | 82.1 ± 1.7 | |
24 | 41.98 ± 1.4 | 24.9 ± 0.8 | 1.04 ± 0.01 | 69.7 ± 12.5 | |
W3110::pI (+) | 6 | 32.51 ± 1.1 | 25.4 ± 1.6 | 4.24 ± 0.07 | 71.1 ± 3.2 |
12 | 40.22 ± 1.7 | 31.9 ± 2.0 | 2.66 ± 0.05 | 89.4 ± 3.5 | |
24 | 42.09 ± 1.9 | 26.3 ± 1.9 | 1.10 ± 0.02 | 73.7 ± 4.2 | |
W3110::pI (−) | 6 | 41.25 ± 1.1 | 36.7 ± 2.0 | 6.11 ± 0.11 | 102.8 ± 2.7 |
12 | 43.85 ± 1.5 | 36.9 ± 1.3 | 3.08 ± 0.09 | 103.4 ± 3.0 | |
24 | 44.69 ± 0.9 | 31.4 ± 2.8 | 1.31 ± 0.02 | 88.0 ± 2.2 |