Fig. 6

Evaluation of the transcript levels of three genes SACE_0528, SACE_1321, and SACE_2475 by RT-qPCR of the control strain WT (white square), WT cultured with 0.4 mmol/L Cu²⁺ (square with upper right to lower left fill), WT-Δ0101 (square with diagonal crosshatch fill), and WT-Δ0101 cultured with 0.4 mmol/L Cu²⁺ (square with horizontal fill). Genes expression in each sample were calculated as the fold expression ratio after normalization to the 16srRNA transcript as a reference control. The values are averages of three independent RT-qPCR experiments; error bars indicate standard deviation