Fig. 7

Characterization of the recombinant CbADH-6M. a Temperature optimum: the enzyme activity was measured at various temperatures (15–75 ℃) in phosphate buffer (pH 7.5). b Thermostability: 1.0 μg/mL purified enzyme was incubated at the specific temperature (50, 60, 70) for different times, and the residual activity was measured by standard assay. c pH optimum: the enzyme activity was assayed in various buffers (6.0–12.5) at 35 ℃. d pH stability: pH stability was measured by incubation of purified enzyme in different buffers (pH 6–10) for 24 h at 35 ℃, and the residual activity was measured by standard assay