Fig. 4From: Redirection of metabolic flux in Shewanella oneidensis MR-1 by CRISPRi and modular design for 5-aminolevulinic acid productionCharacterization of different targeting sites on hemB genes in MR-1. a Several targeting sites for dCas9 were designed on hemB2 (SO_4208). The growth curve (b) and relative ALA yield (c) were analyzed from the strains in LB medium at 12Â h. The bar in c shows the relative ALA yield compared with that in wild-type MR-1. Red dots indicate the OD600 of cellBack to article page