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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Redirection of metabolic flux in Shewanella oneidensis MR-1 by CRISPRi and modular design for 5-aminolevulinic acid production

Fig. 5

Co-expression of ALAS and GroELS in a single plasmid in MR1::T7R with pta and ackA downregulation in MM9 media. a Protein expression was observed by SDS-PAGE at 12 h. The arrows indicate ALAS and GroEL at sizes of approximately 48 kDa and 63 kDa, respectively. S soluble protein, P insoluble protein, M protein ladder marker. b ALA production and biomass in terms by OD600were analyzed at 12 h and 24 h, respectively

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