Fig. 1
From: Identification of an anti-plant-virus molecule in Alpinia zerumbet
Antiviral activities of fractions of A. zerumbet extract separated by reverse-phase chromatography. The concentration of each fraction used in the application of samples to N. benthamiana leaves was adjusted to be the concentration of the original solution. N. benthamiana leaves were inoculated with GFP-tagged ToMV, and treated with extract samples or fractions separated by a C18 Sep-Pak® cartridge. A GFP foci formed at 3 days post-inoculation (dpi). Images were taken under blue light irradiation using the ChemiDoc™ MP Imaging System (Bio-rad, Hercules, CA, USA). B The GFP spots formed on the inoculated leaves were counted at 3 dpi. Protective value (%) = (1 − number of GFP spots on the treated plants/number of GFP spots on the control plants) × 100. Different letters (a and b) indicate significant differences between the control and treated plants at each leaf position by one-way ANOVA with the Tukey–Kramer method (p < 0.05)