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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Production of (R)-mandelic acid from styrene, L-phenylalanine, glycerol, or glucose via cascade biotransformations

Fig. 1

Construction of E. coli cells co-expressing multiple enzymes for the bioproduction of (R)-MA. a E. coli (P2S2A) strain harbouring plasmid pET-PAL-PAD encoding PAL and PAD, plasmid pCDF-SMO-StEH encoding SMO and StEH, and plasmid pRSF-AldO encoding AldO for the conversion of L-phenylalanine to (R)-MA. b E. coli NST74 (P2S2A) strain harbouring plasmid pET-PAL-PAD encoding PAL and PAD, plasmid pCDF-SMO-StEH encoding SMO and StEH, and plasmid pRSF-AldO encoding AldO for the conversion of glycerol or glucose to (R)-MA. c Coupled E. coli NST74-Phe strain over-expressing five limiting enzymes in Shikimate pathway with E. coli (P2S2A) expressing five-enzyme artificial cascade for the conversion of glycerol or glucose to (R)-MA. d SDS-PAGE. Lane M: protein marker; Lane A: total intracellular protein of E. coli NST74; Lane B: total intracellular protein of E. coli NST74 (P2S2A)

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