Carbohydrate-binding modules targeting branched polysaccharides: overcoming side-chain recalcitrance in a non-catalytic approach

Liu, Jiawen; Sun, Di; Zhu, Jingrong; Liu, Cong; Liu, Weijie

doi:10.1186/s40643-021-00381-7

Bioresources and Bioprocessing

Table 1 Available information of CBMs involved in the proposed four-type classification

From: Carbohydrate-binding modules targeting branched polysaccharides: overcoming side-chain recalcitrance in a non-catalytic approach

Name	Modular architecture	Enzyme type	Ligands	Specificity	ABC classification	Four-type classification	References
TmCBM27	GH5-CBM27	Mannanase	Mannan	Linear	Type B	Type I	Boraston et al. (2003)
CcCBM17	GH5-CBM17	Cellulase	β-Glucans/xyloglucan	Linear	Type B	Type I	Najmudin et al. (2006)
BspCBM28	GH5-CBM17-CBM28	Cellulase	β-Glucans/xyloglucan	Linear	Type B	Type I	Najmudin et al. (2006)
CBM79-1_RfGH9	GH9-CBM79-CBM79	Cellulase	β-Glucans/xyloglucan	Linear	Type B	Type I	Venditto et al. (2016)
CBM48	CBM41-CBM48-GH13	Pullulanase	Amylose/amylopectin	Linear	Type B	Type I	Hedin et al. (2017)
RtCBM11	GH26-GH5-CBM11	Cellulase	β-Glucans/xyloglucan	Linear	Type B	Type I	Furtado et al. (2018)
Man5C-CBM35	CBM5-CBM10-CBM35-GH5	Mannanase	Mannan	Similar^a	Type B	Type II	Bolam et al. (2004)
SoCBM13	GH10-CBM13	Xylanase	Xylan	Similar^b	Type B	Type II	Fujimoto et al. (2004)
CBM30, CBM44	CBM30-GH9-GH44-CBM44	Cellulase	β-Glucan/xyloglucan	Similar	Type B	Type II	Najmudin et al. (2006)
CfCBM4-1	CBM4-CBM4-GH9	Cellulase	β-Glucan/xyloglucan	Similar	Type B	Type II	Najmudin et al. (2006)
PeCBM29	Cellulosome	Cellulosome	β-Glucans/xyloglucan	Branched	Type B	Type III	Najmudin et al. (2006)
Unnamed	GH30-CBM35-CBM6	Xylanase	Methylglucuronoxylan	Branched^b	Type B	Type III	St John et al. (2011)
X-2 L110F	CBM4-CBM4-GH10	Xylanase	β-Glucans/xyloglucan	Branched	Type B	Type III	von Schantz et al. (2012)
CBM65A, CBM65B	CBM65-GH5-CBM65-GH5	Cellulase	β-Glucan/xyloglucan	Branched	Type B	Type III	Luis et al. (2013)
CBM75_RfGH43	GH43-CBM75	Arabinofuranosidase	β-Glucans/xyloglucan	Branched	Type B	Type III	Venditto et al. (2016)
CBM76_RfGH44	GH44-CBM76	Cellulase	β-Glucans/xyloglucan	Branched	Type B	Type III	Venditto et al. (2016)
CBM78_RfGH5	GH5-CBM78	Cellulase	β-Glucans/xyloglucan	Branched	Type B	Type III	Venditto et al. (2016)
CBM80_RfGH5-1/2	GH5-CBM80-GH5	Cellulase/mannanase	β-Glucans/xyloglucan	Branched	Type B	Type III	Venditto et al. (2016)
StCBM64C	GH10-CBM64	Xylanase	β-Glucans/xyloglucan	Branched	Type A	Type III	Pires et al. (2017)
Stbd1CBM	CBM20	Human starch-binding domain containing protein 1	Amylopectin/amylose	Branched	Type B	Type III	Skurat et al. (2017)
RtCBM11mut	CBM11-GH12	Xyloglucanase	β-Glucans/xyloglucan	Branched	Type B	Type III	Furtado et al. (2018)
AkCBM42	GH54-CBM42	Arabinofuranosidase	Arabinofuranose	Branched	Type C	Type IV	Miyanaga et al. (2006)
Xyl-CBM35	GH10-CBM35	Xylanase	Glucuronic acid	Branched	Type C	Type IV	Montanier et al. (2009)
CtCBM35-Gal	GH39-CBM35-CBM35	Unknown	α-d-galactose	Branched	Type C	Type IV	Correia et al. (2010)
BsCBM66	GH32-CBM66	β-Fructosidase	Fructosides	Branched	Type C	Type IV	Cuskin et al. (2012)
Laminin_G_3	CBM42-GH43- CBM42-GH43-GH43	Arabinofuranosidase	Arabinooligosaccharide	Branched	Type C	Type IV	Sakka et al. (2019)

^aSimilar indicates that affinity for linear and branched ligands differs by less than 100%
^bSpecificity is inferred based on its binding characteristics and structural information

Back to article page