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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Simultaneous degradation of two mycotoxins enabled by a fusion enzyme in food-grade recombinant Kluyveromyces lactis

Fig. 2

Construction and expression of fusion enzyme ZPF1, and the degradation efficiency of AFB1 and ZEN by ZFP1. a The electrophoresis of the PCR results of the 3 positive transformants: M: DNA marker; L1 to L3: PCR results of 3 positive GG799(pKLAC1-ZPF1) transformants. b SDS-PAGE results of culture supernatants of the 3 positive transformants: M: Protein marker; L1: K. lactis GG799 supernatant proteins; L2: K. lactis GG799(pKLAC1) supernatant proteins; L3 to L5: Three K. lactis GG799(pKLAC1-ZPF1) transformants’ supernatant protein. c Degradation results of AFB1 (reaction system 1) and ZEN (reaction system 2) by the 3 transformants. Different letters above the histogram are significant differences (p < 0.05)

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