Fusion protein expressed in E. coli | Downstream purification schemes (purification steps arranged in sequence) | References | |||||||
---|---|---|---|---|---|---|---|---|---|
rhPI | IB recovery | IMAC | ⭮ | ✁ | IEX | RP | Astolfi et al. (2004) | ||
rhPI | IB recovery | HIC (+ ⭮) | Bai et al. (2003) | ||||||
rhPI | IB recovery | pH-PPT | AEX | pH-PPT | ⭮ | RP | ✁ | RP | Castellanos-Serra et al. (1996) |
rhPI | IB recovery | AEX | ⭮ | RP | Cowley and Mackin (1997) | ||||
rhPI | IB recovery | MMC | Cryst | ⭮ | ✁ | CEX | GE app notes 28-9966-22 AA (2012), 29-0018-56 AB (2012), Heldin et al. (2014) | ||
rhPI | IB recovery | pH-PPT | ⭮ | pH-PPT | ✁ | Kim et al. (2015) | |||
rhPI | ✁ | IEX | RP | SEC | Kroeff et al. (1989) | ||||
rhPI | IB recovery | AC | ⭮ | RP | Mackin (1999) | ||||
rhPI | IB recovery | ⭮ | RP | ✁ | Mackin and Choquette (2003) | ||||
rhPI | IB recovery | pH-PPT | ⭮ | pH-PPT | ✁ | Min et al. (2011) | |||
rhPI | IB recovery | ⭮ | AC | ✁ | RP | Nilsson et al. (1996) | |||
rhPI | IB recovery | CEX | ⭮ | HIC | ✁ | IEX | RP | SEC | Petrides et al. (1995) |
rhPI | IB recovery | IMAC | ZnCl2-PPT | CEX | ⭮ | ✁ | Redwan et al. (2007) | ||
rhPI | IB recovery | IMAC | Yuan et al. (2015) | ||||||
rhPI | IB recovery | SEC (+ ⭮) | Yuan et al. (2015) | ||||||
rhPI | IB recovery | ⭮ | ✁ | AEX | ZnCl2-PPT | AEX | ✁ | RP | Zieliński et al. (2019) |
rhPI, analogues | ✁ | CEX | Coleman et al. (2019) | ||||||
rhPI, analogues | IB recovery | ⭮ | AEX | CEX | Thurow et al. (2010) | ||||
rhPI, analogues | IB recovery | AEX | IMAC | Tikhonov et al. (2001) | |||||
rhPI, analogues | IB recovery | IMAC | AEX | Tikhonov et al. (2001) | |||||
rhPI, analogues | ✁ | MMC | RP | Watson et al. (2018) | |||||
rhPI, analogues | IB recovery | ⭮ | ✁ | AEX | AEX | ✁ | RP | Mikiewicz et al. (2017) | |
PI analogues | IB recovery | ⭮ | IMAC | ✁ | RP | Zimmerman and Stokell (2010) | |||
PI aspart | IB recovery | ⭮ | AEX | Chen et al. (2016) | |||||
PI glargine | IB recovery | ⭮ | pH-PPT | ✁ | CEX | RP | Hwang et al. (2016) |
Molecule | ||
---|---|---|
Inclusion body (IB) | Precursor of human insulin/insulin analogue | Human insulin/insulin analogue |
Chromatography | ||
---|---|---|
AC: affinity chromatography | HIC: hydrophobic interaction chromatography | MMC: mixed-mode chromatography |
AEX: anion exchange chromatography | IEX: ion-exchange chromatography | RP: reversed-phase chromatography |
CEX: cation exchange chromatography | IMAC: immobilized metal ion affinity chromatography | SEC: size exclusion chromatography |