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Table 1 The E. coli strains and plasmids used in this study

From: Ferulic acid production by metabolically engineered Escherichia coli

Strain and plasmid Description Source
JM109(DE3) endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB  +  Δ(lac-proAB) e14-[F′ traD36 proAB  +  lacIq lacZΔM15] hsdR17(rK-Mk  +)  +  λ(DE3) Lab stock
DH10B F-mcrA ∆ (mrr-hsdRMS-mcrBC) φ80lacZ∆M15∆lacX74 recA1endA1 araD139 (ara, leu)7697 galU galK-rpsL nupG Lab stock
pET21d-tal pET21d derived, T7 Prom-tal-T7 Term Lab stock
pET21a-sam5 pET21a derived, T7 Prom-sam5-T7 Term This study
pET21a-comt pET21a derived, T7 Prom-comt-T7 Term This study
pET21d-tal-sam5 pBR322 ori, Amp resistance, T7 Prom-tal-sam5-T7 Term This study
pET21d-tal-sam5-comt pBR322 ori, Amp resistance, T7 Prom-tal-sam5-comt-T7 Term This study
pJF25 pBR322 ori, Apr resistance, T7 Promoter (Wang et al. 2013)
pHJ345 pBR322 ori, Apr resistance, T5 Promoter This study
pHJ352 P15a ori, Apr resistance, T7 Promoter This study
pHJ354 P15a ori, Apr resistance, T5 Promoter This study
pBR322-T7-tal-sam5-comt pBR322 ori, Apr resistance, T7 Prom-tal-sam5-comt-T7 Term This study
pBR322-T5-tal-sam5-comt pBR322 ori, Apr resistance, T5 Prom-tal-sam5-comt-T7 Term This study
p15a-T7-tal-sam5-comt P15a ori, Apr resistance, T7 Prom-tal-sam5-comt-T7 Term This study
p15a-T5-tal-sam5-comt P15a ori, Apr resistance, T5 Prom-tal-sam5-comt-T7 Term This study
pCL1920 pSC101 ori, spectinomycin resistance, cloning vector (Lerner and Inouye 1990)
pCL1920-T7 pSC101 ori, spectinomycin resistance, T7 Prom-MCS-T7 Term Lab stock
pCL1920-T7-Ecicd pSC101 ori, spectinomycin resistance, T7 Prom-Ecicd-T7 Term This study
pCL1920-T7-Ecgnd pSC101 ori, spectinomycin resistance, T7 Prom-Ecgnd-T7 Term This study
pCL1920-T7-Eczwf pSC101 ori, spectinomycin resistance, T7 Prom-Eczwf-T7 Term This study
pCL1920-T7-EcpntAB pSC101 ori, spectinomycin resistance, T7 Prom- EcpntAB-T7 Term This study
pCL1920-T7-CagapN pSC101 ori, spectinomycin resistance, T7 Prom-CagapN-T7 Term This study