Table 1 The E. coli strains and plasmids used in this study
From: Ferulic acid production by metabolically engineered Escherichia coli
Strain and plasmid | Description | Source |
|---|---|---|
JM109(DE3) | endA1 glnV44 thi-1 relA1 gyrA96 recA1 mcrB + Δ(lac-proAB) e14-[F′ traD36 proAB + lacIq lacZΔM15] hsdR17(rK-Mk +) + λ(DE3) | Lab stock |
DH10B | F-mcrA ∆ (mrr-hsdRMS-mcrBC) φ80lacZ∆M15∆lacX74 recA1endA1 araD139 (ara, leu)7697 galU galK-rpsL nupG | Lab stock |
pET21d-tal | pET21d derived, T7 Prom-tal-T7 Term | Lab stock |
pET21a-sam5 | pET21a derived, T7 Prom-sam5-T7 Term | This study |
pET21a-comt | pET21a derived, T7 Prom-comt-T7 Term | This study |
pET21d-tal-sam5 | pBR322 ori, Amp resistance, T7 Prom-tal-sam5-T7 Term | This study |
pET21d-tal-sam5-comt | pBR322 ori, Amp resistance, T7 Prom-tal-sam5-comt-T7 Term | This study |
pJF25 | pBR322 ori, Apr resistance, T7 Promoter | (Wang et al. 2013) |
pHJ345 | pBR322 ori, Apr resistance, T5 Promoter | This study |
pHJ352 | P15a ori, Apr resistance, T7 Promoter | This study |
pHJ354 | P15a ori, Apr resistance, T5 Promoter | This study |
pBR322-T7-tal-sam5-comt | pBR322 ori, Apr resistance, T7 Prom-tal-sam5-comt-T7 Term | This study |
pBR322-T5-tal-sam5-comt | pBR322 ori, Apr resistance, T5 Prom-tal-sam5-comt-T7 Term | This study |
p15a-T7-tal-sam5-comt | P15a ori, Apr resistance, T7 Prom-tal-sam5-comt-T7 Term | This study |
p15a-T5-tal-sam5-comt | P15a ori, Apr resistance, T5 Prom-tal-sam5-comt-T7 Term | This study |
pCL1920 | pSC101 ori, spectinomycin resistance, cloning vector | (Lerner and Inouye 1990) |
pCL1920-T7 | pSC101 ori, spectinomycin resistance, T7 Prom-MCS-T7 Term | Lab stock |
pCL1920-T7-Ecicd | pSC101 ori, spectinomycin resistance, T7 Prom-Ecicd-T7 Term | This study |
pCL1920-T7-Ecgnd | pSC101 ori, spectinomycin resistance, T7 Prom-Ecgnd-T7 Term | This study |
pCL1920-T7-Eczwf | pSC101 ori, spectinomycin resistance, T7 Prom-Eczwf-T7 Term | This study |
pCL1920-T7-EcpntAB | pSC101 ori, spectinomycin resistance, T7 Prom- EcpntAB-T7 Term | This study |
pCL1920-T7-CagapN | pSC101 ori, spectinomycin resistance, T7 Prom-CagapN-T7 Term | This study |