Table 1 Primers used in this study
From: Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine
Primers name | Sequences (5′ → 3′) |
|---|---|
5′AOX1 | GACTGGTTCCAATTGACAAGC |
3′AOX1 | GCAAATGGCATTCTGACATCC |
His4-BamHI-F | GCATGGATCCATGACATTTCCCTTGCTAC |
His4-BamHI-R | GCATGGATCCGTTAAATAAGTCCCAGTTTCTC |
pMPICZA-3881-F | GGTTTAGTTCCTCACCTTG |
pMPICZA-4256-R | TCCCCCTTTTCCTTTGTC |
Kan-pMPICZA-F | ACAAGGTGAGGAACTAAACCATGAGCCATATTCAACGG |
Kan-pMPICZA-R | TCGACAAAGGAAAAGGGGGATTAGAAAAACTCATCGAGC |
Hyg-pMPICZA-F | ACAAGGTGAGGAACTAAACCATGAAAAAGCCTGAACTCAC |
Hyg-pMPICZA-R | TCGACAAAGGAAAAGGGGGACTATTCCTTTGCCCTCGG |
pTEF1-F | GCAATCTAATCTAAGGGGCGGTGT |
CYC1TT-R | TTCGAGCGTCCCAAAACCTTCTCAAGC |
ADCBs-XhoI-F | GCATCTCGAGATGGATGTATCGAACAATGATGAGC |
ADCBs-NotI-R | GCATGCGGCCGCCTACAAAATTGTACGGGCTG |
Oan-XhoI-F | GCATCTCGAGATGTCCGTATCTGAAACC |
Oan-NotI-R | GCATGCGGCCGCTCAGATAACGGTAGTTGCTACGC |
Spe-XhoI-F | GCATCTCGAGATGAAAAAAATCATGATGATCGG |
Spe-NotI-R | GCATGCGGCCGCTTATGCGATAAAGCCACCGTC |
AAT1-BstBI(gb)-F | ATCAAAAAACAACTAATTATTCGAAATGTCGTTTTCAACGCAGAA |
AAT1-NotI(gb)-R | AGTTTTTGTTCTAGAAAGCTGGCGGCCGCCTATACACGCACAACCTGAT |
AAT2-BstBI(gb)-F | ATCAAAAAACAACTAATTATTCGAAATGTCGTTTTCAACGCAGAA |
AAT2-NotI(gb)-R | AGTTTTTGTTCTAGAAAGCTGGCGGCCGCCTATACACGCACAACCTGAT |
AsPCK-BstBI(gb)-F | ATCAAAAAACAACTAATTATTCGAAATGACTGACTTAAACAAACTCG |
AsPCK-NotI(gb)-R | AGTTTTTGTTCTAGAAAGCTGGCGGCCGCTTATGCTTTTGGACCGGCGCCAAC |
PPC-BstBI(gb)-F | ATCAAAAAACAACTAATTATTCGAAATGAACGAACAATATTCCGC |
PPC-NotI(gb)-R | AGTTTTTGTTCTAGAAAGCTGGCGGCCGCTTAGCCGGTATTACGCATAC |
PCK-BstBI(gb)-F | ATCAAAAAACAACTAATTATTCGAAATGCGCGTTAACAATGGTTTG |
PCK-NotI(gb)-R | AGTTTTTGTTCTAGAAAGCTGGCGGCCGCTTACAGTTTCGGACCAGCCGC |