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Table 2 Plasmids and strains constructed in this study

From: Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine

Names Relative characteristics References
Plasmids   
pPICZA Vector for extracellular expression recombinant protein carrying ZeoR Invitrogen
pAOα Vector for extracellular expression; derived from pPICZαA and pAO815, HIS4 +  Zhu et al. (2011)
pMPICZHis Extracellular expression vector carrying HIS4 gene, ZeoR This work
pMPICKmHis Replace antibiotic resistance marker (Zeocin) of pMPICZHis with kanamycin This work
pMPIC2H Replace antibiotic resistance marker (Zeocin) of pMPICZHis with hygromycin This work
pMPICZHis-bliADC Codon-optimized panD gene from Bacillus paralicheniformis ATCC 9945a cloned into pMPICZHis at BstBI and NotI site This work
pMPICZHis-bsADC Codon-optimized panD gene from Bacillus subtilis cloned into pMPICZHis at BstBI and NotI site This work
pMPICZHis-sgeADC Codon-optimized panD gene from Streptomyces griseorubiginosus cloned into pMPICZHis at BstBI and NotI site This work
pMPICZHis-cguADC Codon-optimized panD gene from Corynebacterium glutamicum cloned into pMPICZHis at BstBI and NotI site This work
pMPICZHis-TcCSADC Cysteine sulfinic acid decarboxylase from Tribolium castaneum (TcCSADC) cloned into pMPICZHis at BstBI and NotI site This work
pMPICZHis-Oan aspDH gene from Ochrobactrum anthropi ATCC 4918 cloned into pMPICZHis at BstBI and NotI site This work
pMPICZHis-Spe aspDH gene from Serratia proteamaculans cloned into pMPICZHis at BstBI and NotI site This work
pMPICZHis-ADC-Oan panD expression cassette (pMPICZHis-ADC digest by SphI and BamHI) cloned into pMPICZHis-Oan at SphI and BglII site This work
pMPICZHis-ADC-Spe panD expression cassette (pMPICZHis-ADC digest by SphI and BamHI) cloned into pMPICZHis-Spe at SphI and BglII site This work
pMPICKmHis-AAT1 Cytosolic aspartate aminotransferase from P. pastoris GS115 cloned into pMPICZHis at BstBI and NotI site This work
pMPICKmHis-AAT2 Mitochondrial aspartate aminotransferase from P. pastoris GS115 cloned into pMPICZHis at BstBI and NotI site This work
pMPICZHis-2ADC Two copies of panD gene expression cassette cloned into pMPICZHis This work
pMPICZHis-2ADC-Spe Two copies of panD gene expression cassette and one copy of SpeaspDH gene expression cassette cloned into pMPICZHis This work
pMPIC2H-PPC ppc gene from Escherichia coli cloned into integration vector pMPIC2H at BstBI and NotI site This work
pMPIC2H-AsPCK pck gene from Actinobacillus succinogenes cloned into integration vector pMPIC2H at BstBI and NotI site This work
pMPIC2H-PCK pck gene from Escherichia coli cloned into integration vector pMPIC2H at BstBI and NotI site This work
Strains   
E. coli DH5α Commercial transformation host for cloning Invitrogen
P. pastoris GS115 Commercial transformation host for cloning; HIS4-, Mut +  Invitrogen
Δku70 GS115, ΔKU70; HIS4 +  This work
BliADC Δku70 harboring pMPICZHis-bliADC This work
BsADC Δku70 harboring pMPICZHis-bsADC This work
SgeADC Δku70 harboring pMPICZHis-sgeADC This work
CguADC Δku70 harboring pMPICZHis-cguADC This work
TcCSADC Δku70 harboring pMPICZHis-TcCSADC This work
ADC-Oan Δku70 harboring pMPICZHis-ADC-Oan This work
ADC-Spe Δku70 harboring pMPICZHis-ADC-Spe This work
ADC-AAT1 Δku70 harboring pMPICZHis-bsADC and pMPICKmHis-AAT1 This work
ADC-AAT2 Δku70 harboring pMPICZHis-bsADC and pMPICKmHis-AAT2 This work
2ADC Δku70 harboring pMPICZHis-2ADC This work
2ADC-Spe Δku70 harboring pMPICZHis-2ADC-Spe This work
PPC Δku70 harboring pMPICZHis-2ADC-Spe and pMPIC2H-PPC This work
AsPCk Δku70 harboring pMPICZHis-2ADC-Spe and pMPIC2H-AsPCK This work
PCK Δku70 harboring pMPICZHis-2ADC-Spe and pMPIC2H-PCK This work