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Table 3 Exploration of recent purification method for enhanced cellulase activity

From: Current perspective on production and applications of microbial cellulases: a review

Strain and cellulase Purification methods S.A (IU) Yield (%) Purification fold Mol. Wt. (kDa) References
Bacillus tequilensis G9 Ammonium sulfate precipitation (70%) 26,505 ± 90 21.7 3.6 43 Dar et al. (2019)
Acetone precipitation 44,602 ± 50 21 3.74
Ion-exchange chromatography technique (DEAE)-cellulose column (25 × 2 cm) equilibrated with 20 mM PBS (pH 7.4)    9.89
Pleurotus ostreatus Ammonium sulphate precipitation 36.8 54 10.5 58 Okereke et al. (2017)
Dialysis 52.0 30.5 14.9
Gel filtration Chromatography 61.0 17.4 17.4
Bacillus subtilis Polyethylene-glycol (PEG)/sodium citrate aqueous biphasic system (ABS) 82.86 4.8 32–46 Ho et al. (2017)
Bacillus sp. H1666 Acetone precipitation followed by ion exchange and size exclusion chromatography   29 66.23 40 Harshvardhan et al. (2013)
Rhizopus stolonifer var. reflexus TP-02 Ammonium sulphate followed by dialysis, ultrafiltration, and Sephadex G-100 121.31   34.96 40–46 Tang et al. (2012)