Table 3 Exploration of recent purification method for enhanced cellulase activity
From: Current perspective on production and applications of microbial cellulases: a review
Strain and cellulase | Purification methods | S.A (IU) | Yield (%) | Purification fold | Mol. Wt. (kDa) | References |
|---|---|---|---|---|---|---|
Bacillus tequilensis G9 | Ammonium sulfate precipitation (70%) | 26,505 ± 90 | 21.7 | 3.6 | 43 | Dar et al. (2019) |
Acetone precipitation | 44,602 ± 50 | 21 | 3.74 | |||
Ion-exchange chromatography technique (DEAE)-cellulose column (25 × 2 cm) equilibrated with 20 mM PBS (pH 7.4) |  |  | 9.89 | |||
Pleurotus ostreatus | Ammonium sulphate precipitation | 36.8 | 54 | 10.5 | 58 | Okereke et al. (2017) |
Dialysis | 52.0 | 30.5 | 14.9 | |||
Gel filtration Chromatography | 61.0 | 17.4 | 17.4 | |||
Bacillus subtilis | Polyethylene-glycol (PEG)/sodium citrate aqueous biphasic system (ABS) | – | 82.86 | 4.8 | 32–46 | Ho et al. (2017) |
Bacillus sp. H1666 | Acetone precipitation followed by ion exchange and size exclusion chromatography | Â | 29 | 66.23 | 40 | Harshvardhan et al. (2013) |
Rhizopus stolonifer var. reflexus TP-02 | Ammonium sulphate followed by dialysis, ultrafiltration, and Sephadex G-100 | 121.31 |  | 34.96 | 40–46 | Tang et al. (2012) |