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Table 3 Summary of the plasmids, DNA constructs, and peptide sequences

From: A hemolysin secretion pathway-based novel secretory expression platform for efficient manufacturing of tag peptides and anti-microbial peptides in Escherichia coli

Backbone DNA constructs Plasmids Peptide sequences
pBAD-gIII B HlyB–HlyD pBAD-BD
pET-28a TEVcs-6*His-HlyA pET-THHly
His tag-TEVcs-6*His-HlyA pET-HTHHly HHHHHH
T7 tag-TEVcs-6*His-HlyA pET-TTHHly MASMTGGQQMG
S tag-TEVcs-6*His-HlyA pET-STHHly KETAAAKFERQHMDS
Flag tag-TEVcs-6*His-HlyA pET-FTHHly DYKDDDDK
Myc tag-TEVcs-6*His-HlyA pET-MTHHly EQKLISEEDL
Cecropin A-TEVcs-6*His-HlyA pET-CeATHHly KWKLFKKIEKVGQNIRDG
IIKAGPAVAVVGQATQIAK
Melittin-TEVcs-6*His-HlyA pET-MelTHHly GIGAVLKVLTTGLPALIS
WIKRKRQQ