Table 3 Summary of the plasmids, DNA constructs, and peptide sequences
Backbone | DNA constructs | Plasmids | Peptide sequences |
|---|---|---|---|
pBAD-gIII B | HlyB–HlyD | pBAD-BD | – |
pET-28a | TEVcs-6*His-HlyA | pET-THHly | – |
His tag-TEVcs-6*His-HlyA | pET-HTHHly | HHHHHH | |
T7 tag-TEVcs-6*His-HlyA | pET-TTHHly | MASMTGGQQMG | |
S tag-TEVcs-6*His-HlyA | pET-STHHly | KETAAAKFERQHMDS | |
Flag tag-TEVcs-6*His-HlyA | pET-FTHHly | DYKDDDDK | |
Myc tag-TEVcs-6*His-HlyA | pET-MTHHly | EQKLISEEDL | |
Cecropin A-TEVcs-6*His-HlyA | pET-CeATHHly | KWKLFKKIEKVGQNIRDG IIKAGPAVAVVGQATQIAK | |
Melittin-TEVcs-6*His-HlyA | pET-MelTHHly | GIGAVLKVLTTGLPALIS WIKRKRQQ |