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Table 2 Multi-stage extraction lactic acid by [Bmim]BF4/glucose systems from synthetic solution and lignocellulosic biomass-derived broth at pH 2.0

From: Ionic liquid-based multi-stage sugaring-out extraction of lactic acid from simulated broth and actual lignocellulosic fermentation broth

Solution Stage

R

K

Y (%)

DM (%)

LA

Glucose

IL

LA

Glucose

IL

Cell

Pigments

Synthetic solution

1st

1.16

0.99

0.02

10.02

53.4

2.7

92.1

–

–

2nd

1.11

0.99

0.02

11.40

52.2

2.5

92.7

–

–

3rd

1.04

1.00

0.02

12.27

50.8

1.8

92.7

–

–

Total

–

–

–

–

89.0

6.8

92.5

–

–

Filtered broth

1st

1.12

0.87

0.02

12.40

49.2

1.8

93.3

84.9

72.8

2nd

1.05

1.06

0.02

23.86

52.8

2.0

95.1

90.9

86.5

3rd

1.08

1.18

0.03

41.15

56.2

3.1

97.4

91.5

90.5

Total

–

–

–

–

89.5

6.6

95.2

70.6

57.0

Unfiltered broth

1st

1.03

0.94

0.02

12.62

49.2

1.7

92.8

83.9

78.5

2nd

1.02

1.10

0.01

33.99

52.1

1.1

97.1

88.8

90.0

3rd

0.95

1.51

0.02

54.04

58.2

1.4

98.0

91.9

92.7

Total

–

–

–

–

89.8

4.1

96.0

68.4

65.4

  1. The synthetic solution and fermentation broth contain 89.25 g/L and 34.32 g/L lactic acid, respectively. 45% (w/w) [Bmim]BF4 and 25% (w/w) glucose were used