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Table 1 E. coli strains and plasmids used in this study

From: Strain engineering and bioprocessing strategies for biobased production of porphobilinogen in Escherichia coli

Name Description or relevant genotype Source
E. coli host strains
 DH5α F − , endA1, glnV44, thi-1, recA1, relA1, gyrA96, deoR, nupG φ80d lacZ∆acZd ladlacZYA – argF) U169, hsdR17(rK-mK +), λ- Lab stock
 BW25113 F-, ∆(araD-araB)567, ∆lacZ4787(::rrnB-3), λ-, rph-1, ∆(rhaD-rhaB)568, hsdR514 (Datsenko and Wanner 2000)
 BW∆ldhA BW25113 ldhA null mutant (Srirangan et al. 2014)
 DMH BW∆ldhA/pK-hemA (Miscevic et al. 2021)
 DMH∆sdhA sdhA null mutant of DMH (Miscevic et al. 2021)
 DMH∆iclR iclR null mutant of DMH This study
 DMH∆iclRsdhA iclR and sdhA mutants of DMH (Miscevic et al. 2021)
 DMH-D9∆sdhA DMH∆sdhA/pK-hemA/pgRNA-D9/pdcas9-bacteria This study
 DMH-D9∆iclRsdhA DMH∆iclRsdhA /pK-hemA/pgRNA-D9/pdcas9-bacteria This study
 DSL BW∆ldhA/pK-hemA-hemB This study
 DSL∆sdhA sdhA null mutant of DSL This study
 DSL∆iclR iclR null mutant of DSL This study
 DSL∆iclRsdhA iclR and sdhA mutants of DSL This study
 DSL-D9∆sdhA DSL∆sdhA/pK-hemA-hemB/pgRNA-D9/pdcas9-bacteria This study
 DSL-D1∆iclRsdhA
 DSL-D2∆iclRsdhA
 DSL-D3∆iclRsdhA
 DSL-D4∆iclRsdhA
 DSL-D5∆iclRsdhA
 DSL-D6∆iclRsdhA
 DSL-D7∆iclRsdhA
 DSL-D8∆iclRsdhA
 DSL-D9∆iclRsdhA
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D1/pdcas9-bacteria
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D2/pdcas9-bacteria
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D3/pdcas9-bacteria
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D4/pdcas9-bacteria
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D5/pdcas9-bacteria
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D6/pdcas9-bacteria
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D7/pdcas9-bacteria
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D8/pdcas9-bacteria
DSL∆iclRsdhA /pK-hemA-hemB/pgRNA-D9/pdcas9-bacteria
This study
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Plasmids
 pCP20
 pK184
Flp + , λ cI857 + , λ pR Rep(pSC101 ori)ts, ApR, CmR
p15A ori, KmR, Plac::lacZ’
(Cherepanov and Wackernagel 1995)
(Jobling and Holmes 1990)
 pdcas9-bacteria p15A ori, PTet-dCas9 (Qi et al. 2013)
 pgRNA-bacteria ColE1 origin, PJ23119-gRNA (Qi et al. 2013)
 pgRNA-D1
 pgRNA-D2
 pgRNA-D3
 pgRNA-D4
 pgRNA-D5
 pgRNA-D6
 pgRNA-D7
 pgRNA-D8
 pgRNA-D9
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D1
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D2
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D3
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D4
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D5
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D6
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D7
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D8
Derived from pgRNA-bacteria, PspeI::hemC-gRNA-D9
This study
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This study
 pK-hemA Derived from pK184, Plac::hemA (Miscevic et al. 2021)
 pK-hemA-hemB Derived from pK184, Plac::hemA-hemB This study