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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Reshaping the binding channel of a novel GH113 family β-mannanase from Paenibacillus cineris (PcMan113) for enhanced activity

Fig. 3

Effects of pH and temperature on the activity of PcMan113. a pH dependence of PcMan113. Activity was measured in the reaction mixtures adjusted to various pH values with glycine–HCl buffer, Na2HPO4–citrate buffer, PBS buffer and Tris–HCl buffer. b Analysis of pH stability of PcMan113. PcMan113 was preincubated at different pH values for 1 h at room temperature, and the residual activity was determined at various pH conditions. c Temperature dependence of PcMan113. Activity was measured at various temperatures in the standard assay conditions. d Thermostability analysis of PcMan113. PcMan113 was preincubated at various temperatures for 30 min. The activities of wild-type PcMan113 are represented as 100 in optimal reaction conditions, error bars are standard deviations (n = 3)

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