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Bioresources and Bioprocessing
Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Immobilization of engineered E. coli cells for asymmetric reduction of methyl acetoacetate to methyl-(R)-3-hydroxybutyrate

Fig. 6

Effects of substrate concentrations on the bio-reduction of MAA to (R)-HBME with alginate-immobilized recombinant E. coli cells. Reaction conditions: 4 mL Tris–HCl buffer (200 mM, pH 7.0), 800 mM glucose, different concentrations of MAA (300, 350, 400, 450, 500, 600, 700 mM), 30 mg/mL free recombination E. coli cells (wet weight), or immobilized cells containing the same amount of free cells, 40 ℃, 200 rpm; (n = 3, different lowercase letters represent significant differences between groups, p < 0.05)

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