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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Enhanced thermal and alkaline stability of L-lysine decarboxylase CadA by combining directed evolution and computation-guided virtual screening

Fig. 3

Computational saturation mutagenesis of the decamer interface of CadA. a The decamer interface region of CadA is a decamer composed of five dimers. F102, E445, and T544 are located in interfacial region A, while D41 is located in interfacial region B. b Experimental verification results of the mutants with improved stability as predicted by virtual screening. The error bars represent standard deviations calculated from triplicate experiments, and statistical significance of the different relative cadaverine yield in comparison with the wild type was evaluated using Student’s t test (*, P \(<\) 0.05, **, P \(<\) 0.01)

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