Fig. 3

a Formation of the BA hydrolysis product in batch conditions (per unit concentration of Trypsin enzyme in the system) using 3D printed hollow cylinders with 7.31 mg/g immobilized trypsin (red) and using free trypsin in solution at a concentration of 2 mg/mL (black). b BA conversion obtained from continuous operation of immobilized enzyme bioreactor with Schoen gyroid bed geometry in steady-state mode at different flow rates. In both cases, the monoliths were equilibrated in 50 mM Tris buffer pH 8, followed by introduction of a 1 mM BAEE substrate solution (in 50 mM Tris buffer pH 8), with UV monitoring of the formation of the hydrolysis product (BA) at 253 nm