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Table 2 Enzymatic phosphorylation of nicotinamide riboside with Klm-NRK

From: Enzymatic synthesis of high-titer nicotinamide mononucleotide with a new nicotinamide riboside kinase and an efficient ATP regeneration system

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Entry

NR (g·L–1)

ATP (g·L–1)

Pi donor

Time (h)

Conv. (%)

1

50

141

ATP

8

99.2

2

75

211

ATP

27

99.2

3

100

282

ATP

27

82.1

4

50

0.114

AcP/AcK

6

99.7

5

50

1.14

AcP/AcK

2

99.7

6

100

1.14

AcP/AcK

8

98.3