Table 1 Comparison of optimum temperatures between bacterial type I pullulanases
Bacterial source | Accession number | Optimum temperature (℃) | Similarity with PulAR | Special activity | References |
|---|---|---|---|---|---|
Bacillus stearothermophilus | 1808262A | 60 | 70.8% | 0.214 U/mL | Kuriki et al. (1990) |
Geobacillus thermoleovorans | CAC85704.1 | 70 | 60.2% | 36 U/mg | Zouari Ayadi et al. (2008) |
Anoxybacillus sp. LM18-11 | AEW23439.1 | 60 | 58.3% | vmax 750 U/mg | Xu et al. (2014) |
Bacillus sp. CICIM 263 | AGA03915.1 | 70 | 46.5% | 73 U/mg | Li et al. (2012) |
Anaerobranca gottschalkii | AAS47565.1 | 65–70 | 43.6% | 56 U/mg | Bertoldo et al. (2004) |
Fervidobacterium pennivorans DSM 9078 | AAD30387.1 | 80 | 41.5% | 78 U/mg | Bertoldo et al. (1999) |
Thermotoga neapolitana | ACN58254.1 | 80–85 | 41.1% | 25.1 U/mg | Kang et al. (2011) |
Caldicellulosiruptor saccharolyticus | AAB06264.1 | 85 | 38.1% | – | Albertson et al. (1997) |
Anoxybacillus sp. AR-2 | KY273924 | 55 | 100.0% | 24.4 U/mg | This study |
KY273924 | 65 | 99.0% | 87.8U/mg (PulAR-A365V/V401C/T504V/H499A) | This study |