Skip to main content
Bioresources and Bioprocessing

Table 3 Half-lives of WT-PulAR and its variants at pH 4.5 and 5.0

From: Tailoring pullulanase PulAR from Anoxybacillus sp. AR-29 for enhanced catalytic performance by a structure-guided consensus approach

Mutant

pH 4.5

pH5.0

kd (1/h)

t1/2 (h)

kd (1/h)

t1/2 (h)

WT-PulAR

0.13

5.4 ± 0.3

0.11

6.1 ± 0.5

PulAR-A365V

0.10

7.0 ± 0.4

0.08

8.5 ± 1.4

PulAR-V401C

0.10

7.1 ± 0.6

0.08

8.4 ± 0.3

PulAR-A365V-V401C

0.07

9.4 ± 0.3

0.06

10.8 ± 0.2

PulAR-A365V-V401C-T504V

0.06

11.1 ± 0.4

0.05

13.6 ± 1.0

PulAR-A365V-V401C-T504V-H499A

0.05

13.9 ± 1.2

0.04

17.3 ± 1.2

Back to article page