Skip to main content
Bioresources and Bioprocessing

Table 3 Specific activity (mU mg−1) of F-BbAmDH and cFLF-AmDH toward aliphatic ketones

From: High coenzyme affinity chimeric amine dehydrogenase based on domain engineering

Substrates

Specific activity (mU mg−1)a

F-BbAmDH

cFLF-AmDH

4-Methyl-2-butanone

n.a.b

n.a

4-Hydroxy-2-butanone

n.a

n.a

2-Pentanone

33.5

143.32

4-Methyl-2-pentanone

n.a

25.29

5-Hydroxy-2-pentanone

n.a

34.75

  1. aActivity was measured in NH4Cl/NH4OH buffer (2 M, pH 9.0) containing 0.2 mM NADH and 20 mM substrate at 30 °C
  2. bn.a. = no measurable activity
Back to article page