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Table 1 Equilibrium dissociation constant (KD) values of the binding affinity of PsPIWI-RE to DNA and RNA oligonucleotides

From: Catalytic properties and biological function of a PIWI-RE nuclease from Pseudomonas stutzeri

Oligonucleotides

KD (nM)

5′P-DNA(5′T)

24.61 ± 1.57

5′P-RNA(5′U)

64.44 ± 3.53

5′OH-DNA(5′T)

19.71 ± 2.52

5′OH-RNA(5′U)

86.50 ± 16.68

5′OH-RNA(5′A)

88.42 ± 14.83

5′OH-RNA(5′C)

152.30 ± 29.00

5′OH-RNA(5′G)

86.74 ± 11.02

  1. The KD values were measured using a fluorescence polarization assay with various nucleic acids