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Table 1 Equilibrium dissociation constant (KD) values of the binding affinity of PsPIWI-RE to DNA and RNA oligonucleotides

From: Catalytic properties and biological function of a PIWI-RE nuclease from Pseudomonas stutzeri

Oligonucleotides KD (nM)
5′P-DNA(5′T) 24.61 ± 1.57
5′P-RNA(5′U) 64.44 ± 3.53
5′OH-DNA(5′T) 19.71 ± 2.52
5′OH-RNA(5′U) 86.50 ± 16.68
5′OH-RNA(5′A) 88.42 ± 14.83
5′OH-RNA(5′C) 152.30 ± 29.00
5′OH-RNA(5′G) 86.74 ± 11.02
  1. The KD values were measured using a fluorescence polarization assay with various nucleic acids