Skip to main content

Table 2 Bacterial strains and plasmids

From: A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis

Strain or plasmid

Characteristics

Source

Bacillus subtilis

 SCK6

ErmR, his nprR2 nprE18 aprA3 eglS102 bglT bglSRV, lacA::PxylA-comK

Lab stock

 SCK8

ErmR, SCK6 derivate, upp

This work

 SCK9

ErmR, SCK8 derivate, uppspoIIAC

This work

 SCK10

ErmR, SCK9 derivate, uppspoIIACsrfAC

This work

 SCK22

ErmR, SCK10 derivate, uppspoIIACsrfAC, amyE::P43-T7RNAP

This work

Plasmids

 pSS

AmpR, CmR, modular vector carrying upp-cassette

Lab stock

 pHT01

AmpR, CmR, E. coli-B. subtilis shuttle vector

Lab stock

 pDG1730

SpcR, integration vector contains a spectinomycin resistance gene sandwiched between amyE-front and amyE-back

Lab stock

 pDG1730-T7RNAP

SpcR, pDG1730 derivate, used to integrate genes on the genome, with T7RNAP expression cassette (spc-upp-P43-T7RNAP) and amyE gene upstream and downstream homology arms

This work

 pHT7

AmpR, CmR, pHT01 derivate, with T7-lac promoter

This work

 pHT7-GFP

AmpR, CmR, pHT7 derivate, with GFP cloned

This work

 pHT7-αGP

AmpR, CmR, pHT7 derivate, with αGP cloned

This work

 pHT7-IMP

AmpR, CmR, pHT7 derivate, with IMP cloned

This work

 pHT7-PGM

AmpR, CmR, pHT7 derivate, with PGM cloned

This work

 pHT7-4GT

AmpR, CmR, pHT7 derivate, with 4GT cloned

This work