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Table 2 Bacterial strains and plasmids

From: A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis

Strain or plasmid Characteristics Source
Bacillus subtilis
 SCK6 ErmR, his nprR2 nprE18 aprA3 eglS102 bglT bglSRV, lacA::PxylA-comK Lab stock
 SCK8 ErmR, SCK6 derivate, upp This work
 SCK9 ErmR, SCK8 derivate, uppspoIIAC This work
 SCK10 ErmR, SCK9 derivate, uppspoIIACsrfAC This work
 SCK22 ErmR, SCK10 derivate, uppspoIIACsrfAC, amyE::P43-T7RNAP This work
Plasmids
 pSS AmpR, CmR, modular vector carrying upp-cassette Lab stock
 pHT01 AmpR, CmR, E. coli-B. subtilis shuttle vector Lab stock
 pDG1730 SpcR, integration vector contains a spectinomycin resistance gene sandwiched between amyE-front and amyE-back Lab stock
 pDG1730-T7RNAP SpcR, pDG1730 derivate, used to integrate genes on the genome, with T7RNAP expression cassette (spc-upp-P43-T7RNAP) and amyE gene upstream and downstream homology arms This work
 pHT7 AmpR, CmR, pHT01 derivate, with T7-lac promoter This work
 pHT7-GFP AmpR, CmR, pHT7 derivate, with GFP cloned This work
 pHT7-αGP AmpR, CmR, pHT7 derivate, with αGP cloned This work
 pHT7-IMP AmpR, CmR, pHT7 derivate, with IMP cloned This work
 pHT7-PGM AmpR, CmR, pHT7 derivate, with PGM cloned This work
 pHT7-4GT AmpR, CmR, pHT7 derivate, with 4GT cloned This work