Table 3 Primers used in this work
From: A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis
Primers | Oligo sequences (5ʹ → 3ʹ) |
|---|---|
Primers used in construction of pHT7-GFP | |
 P1 | gtttaactttaagaaaggaggatataccatgagtaaaggagaagaacttttc |
 P2 | gctttgttagcagccggatctcattatttgtatagttcatccatgccatg |
 P3 | catggcatggatgaactatacaaataatgagatccggctgctaacaaagc |
 P4 | gaaaagttcttctcctttactcaggtatatcctcctttcttaaagttaaac |
Primers used in construction of pHT7-αGP | |
 P5 | ctttaagaaaggaggatataccatggtgaacgtttccaatgccgttgaggatg |
 P6 | cgggctttgttagcagccggatcttagtcaagtcccttccacttgaccagac |
 P7 | catcctcaacggcattggaaacgttcaccatggtatatcctcctttcttaaag |
 P8 | gtctggtcaagtggaagggacttgactaagatccggctgctaacaaagcccg |
Primers used in construction of pHT7-IMP | |
 P9 | taactttaagaaaggaggatataccatgctggatcgcctggatttctctattaaactgctgcg |
 P10 | cgggctttgttagcagccggatctcatttaccgccgatttcttcaacaac |
 P11 | taactttaagaaaggaggatataccatgctggatcgcctggatttctctattaaactgctgcg |
 P12 | gttgttgaagaaatcggcggtaaatgagatccggctgctaacaaagcccg |
Primers used in construction of pHT7-PGM | |
 P13 | ctttaagaaaggaggatataccatggggaagctgtttggaacatttggag |
 P14 | cgggctttgttagcagccggatcttatgaaagcgctttctcaagtagctc |
 P15 | gagctacttgagaaagcgctttcataagatccggctgctaacaaagcccg |
 P16 | ctccaaatgttccaaacagcttccccatggtatatcctcctttcttaaag |
Primers used in construction of pHT7-4GT | |
 P17 | ctttaagaaaggaggatataccatggaacgtatcaacttcatcttcggtatcc |
 P18 | ggctttgttagcagccggatctcacagttcgcgaaaacgaacggtgaattcc |
 P19 | ggaattcaccgttcgttttcgcgaactgtgagatccggctgctaacaaagcc |
 P20 | ggataccgaagatgaagttgatacgttccatggtatatcctcctttcttaaag |