Table 1 Strains and plasmids used in this study
Name | Strains | Genotype | Source |
|---|---|---|---|
DH5α | E. coli DH5α | endA1, hsdR17 (rk−, mk+), phoA, supE44λ−, thi-1, gyrA96, relA1 | Invitrogen |
Suc260 | E. coli Suc260 | Adaptive mutant of E. coli BER208 | Zhang et al. (2018a) |
G0 | E. coli Suc360 | Suc260/pTrc99A | Zhang et al. (2018a) |
G1 | E. coli Suc361 | Suc260/pTrc99A/mdh2 | This study |
G2 | E. coli Suc362 | Suc260/pTrc99A/mdh3 | This study |
G3 | E. coli Suc363 | Suc260/pTrc99A/mdh2/fdhABCD | This study |
G4 | E. coli Suc364 | Suc260/pTrc99A/mdh2/fdh1 | This study |
G5 | E. coli Suc365 | Suc260/pTrc99A/mdh2/fdh1/pyc | This study |
Plasmid | |||
 pTrc99A | AmpR, Ptrc, pBR322_origin | Invitrogen | |
 pUC18-mdh2 | AmpR, Plac, pBR322_origin | Genscript | |
 pUC18-mdh3 | AmpR, Plac, pBR322_origin | Genscript | |
 pTrc99A-mdh2 | AmpR, Ptrc, B. methanolicus mdhB | This study | |
 pTrc99A-mdh3 | AmpR, Ptrc, B. stearothermophilus mdh | This study | |
 pTrc99A-fdhABCD | AmpR, Ptrc, M. extorquens fdhABCD | This study | |
 pTrc99A-mdh2-fdh1 | AmpR, Ptrc, B. methanolicus mdh, C. boidinii fdh | This study | |
 pTrc99A-mdh2-fdhABCD | AmpR, Ptrc, B. methanolicus mdh, M. extorquens fdhABCD | This study | |
 plgz920-pyc | AmpR, Ppck, 130Z_origin | (Liang et al. 2012) | |
 pTrc99A-pyc | AmpR, Ptrc, Lactococcus lactis pyc | Genscript | |
 pTrc99A-mdh1-fdh1-pyc | AmpR, Ptrc, B. methanolicus mdh, C. boidinii fdh, Lactococcus lactis pyc | This study | |