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Bioresources and Bioprocessing

Table 1 Steady-state kinetic parameters of bFMO and the mutants toward indole

From: Structure-guided engineering of a flavin-containing monooxygenase for the efficient production of indirubin

Enzyme

Indole

kcat (s−1)

Km (mM)

kcat/Km (s−1 mM−1)

Fold

WT

0.22 ± 0.01

0.98 ± 0.12

0.22 ± 0.02

1

N291T

0.45 ± 0.04

1.14 ± 0.30

0.45 ± 0.08

2.0

K223R

0.55 ± 0.04

0.75 ± 0.19

0.55 ± 0.08

2.5

K223R/D317S

0.79 ± 0.03

0.55 ± 0.14

1.45 ± 0.30

6.6

K223R/D317M

0.30 ± 0.03

1.61 ± 0.43

0.18 ± 0.03

0.8

K223R/D317A

0.16 ± 0.02

1.12 ± 0.31

0.14 ± 0.03

0.6

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