Fig. 2

Optimization of the kind (a) and equivalents (b) of amino donor in Module 2. Reaction conditions (0.5 mL): shaken at 35 °C, 800 rpm for 12 h, KPB buffer (pH 7.5, 100 mM). The reaction mixture (0.5 mL) was composed of 10 mM (−)-perillyl alcohol (with 2% DMSO), 0.2 U/mL LkADH, 0.4 U/mL SmNOX, 2 U/mL ATA-117, 0.2 mM PLP, 0.2 mM NAD⁺, in addition to: a 50 mM DL-Ala/IPA/2-pentanamine; b 10−100 mM 2-pentanamine. IPA isopropanyl alcohol, Concn. Concentration, 2-PTAM 2-pentanamine, equiv. equivalent