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Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Bioeconomic production of high-quality chitobiose from chitin food wastes using an in-house chitinase from Vibrio campbellii

Fig. 5

Purification of (GlcNAc)2 product. a Reaction sample before passage through a gel filtration column packed with cellulose beads (Cellufine, lot No. R2407, JNC Corporation, Japan) column. b Reaction sample after gel filtration. Sugar products were detected at 200 nm. DP1, DP2, and DP3 represent GlcNAc, (GlcNAc)2 and (GlcNAc)3, respectively. c (GlcNAc)2 was eluted from a preparative NH2P-50 10E column, by acetonitrile water mixture (70:30 v/v) and detected in a PDA mode 200 nm. The retention time of the product was compared with those of standard sugars (data not shown). d Product characterization of chitobiose by QTOF-MS

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