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Bioresources and Bioprocessing
Fig. 8 | Bioresources and Bioprocessing

Fig. 8

From: Two strategies to improve the supply of PKS extender units for ansamitocin P-3 biosynthesis by CRISPR–Cas9

Fig. 8

Analysis of intracellular TAG and M-CoA in PKS-disrupted mutants. A Relative levels of intracellular TAG pool of strain MD02, MD04, MD15 on day 2 and day 3 of fermentation. Data were normalized based on the TAG pool of strain L40 at day 2 of fermentation. The values are means ± SD of three independent experiments. Significant differences were analyzed by two-way ANOVA, and ****p < 0.0001. B Concentrations of the intracellular M-CoA in gene cluster-disrupted mutants. The values are means ± SD of three independent experiments. L40 the parent strain. MD02 mutant with T1PKS-15 deletion. MD04 mutant with T1PKS-18 deletion. MD15 mutant with T1PKS-15 and T1PKS/NRPS-5 deletion

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