Table 2 Summary of the recombinant S. cerevisiae strains used in this study
From: An accessory enzymatic system of cellulase for simultaneous saccharification and co-fermentation
Plasmids or Strain | Genotype/property | Resource |
|---|---|---|
S. cerevisiae | ||
W303-1A | MATa ade2 trp1 his3 can1 ura3 leu2 | |
WA1 | W303-1A derivative, {PGK1p-Aga1-Leu2-Matt}, integrated into the chromosome expressing Aga1 in cell surface | Tian, 2019 |
WA1δ/XR-XDH | WA1 derivative, {PGK1p-XYL1-Matt; PGK1p-XYL2-Matt}, based on traditional delta integration | This work |
WA1g/XR-XDH | WA1 derivative, {PGK1p-XYL1-Matt; PGK1p-XYL2-Matt}, based on delta integration enhanced by CRISPR/Cas9 | This work |
WA1g/ScafI | WA1g/XR-XDH derivative, surface displaying the achoring scaffoldin ScafI | This work |
WA1g/XA | WA1g/XR-XDH derivative, expression of catalytical module XylA containg dockerin OlpA | This work |
WA1g/Xn | WA1g/XR-XDH derivative, expression of catalytical module XynII containg dockerin SdbA | This work |
WA1g/NC | WA1g/XR-XDH derivative, was transferred plasmid PYD1 as control | This work |
Plasmids | ||
YD1 | Ori, GAL1p, Aga2, Matt, Amp, Trp3 | Commercial |
pYD1–PGK–aga2 | pYD1, PGK1p, Aga2, Matt, Amp, Trp3 | Tian, 2019 |
pYD1–PGK–αMF | pYD1, PGK1p, αMF, Matt, Amp, Trp3 | Tian, 2019 |
pCRCT | Ura3, encoding iCas9, tracrRNA and crRNAs | Commercial |
pDi-g1 | pCRCT, gRNA targeting δ sequence | This work |
pYD1–ScafI | pYD1, PGK1p-Aga2-ScafI-Matt, Amp, Trp3 | This work |
pYD1–XylA | pYD1, PGK1p-αMF-XylA-doc-OlpA-Matt, Amp, Trp3 | This work |
pYD1–XynII | pYD1, PGK1p-αMF-XynII-doc-SdbA-Matt, Amp, Trp3 | This work |