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Table 2 Summary of the recombinant S. cerevisiae strains used in this study

From: An accessory enzymatic system of cellulase for simultaneous saccharification and co-fermentation

Plasmids or Strain Genotype/property Resource
S. cerevisiae
 W303-1A MATa ade2 trp1 his3 can1 ura3 leu2  
 WA1 W303-1A derivative, {PGK1p-Aga1-Leu2-Matt}, integrated into the chromosome expressing Aga1 in cell surface Tian, 2019
 WA1δ/XR-XDH WA1 derivative, {PGK1p-XYL1-Matt; PGK1p-XYL2-Matt}, based on traditional delta integration This work
 WA1g/XR-XDH WA1 derivative, {PGK1p-XYL1-Matt; PGK1p-XYL2-Matt}, based on delta integration enhanced by CRISPR/Cas9 This work
 WA1g/ScafI WA1g/XR-XDH derivative, surface displaying the achoring scaffoldin ScafI This work
 WA1g/XA WA1g/XR-XDH derivative, expression of catalytical module XylA containg dockerin OlpA This work
 WA1g/Xn WA1g/XR-XDH derivative, expression of catalytical module XynII containg dockerin SdbA This work
 WA1g/NC WA1g/XR-XDH derivative, was transferred plasmid PYD1 as control This work
Plasmids
 YD1 Ori, GAL1p, Aga2, Matt, Amp, Trp3 Commercial
 pYD1–PGK–aga2 pYD1, PGK1p, Aga2, Matt, Amp, Trp3 Tian, 2019
 pYD1–PGK–αMF pYD1, PGK1p, αMF, Matt, Amp, Trp3 Tian, 2019
 pCRCT Ura3, encoding iCas9, tracrRNA and crRNAs Commercial
 pDi-g1 pCRCT, gRNA targeting δ sequence This work
 pYD1–ScafI pYD1, PGK1p-Aga2-ScafI-Matt, Amp, Trp3 This work
 pYD1–XylA pYD1, PGK1p-αMF-XylA-doc-OlpA-Matt, Amp, Trp3 This work
 pYD1–XynII pYD1, PGK1p-αMF-XynII-doc-SdbA-Matt, Amp, Trp3 This work