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Table 3 Effect of elicitation time combined with 200 mg/L YE or 150 mg/L MeJA on cell growth parameters and costunolide accumulation

From: Effect of explant type (leaf, stem) and 2,4-D concentration on callus induction: influence of elicitor type (biotic, abiotic), elicitor concentration and elicitation time on biomass growth rate and costunolide biosynthesis in gazania (Gazania rigens) cell suspension cultures

Treatments Fresh weight (mg) Dry weight (mg) Costunolide (ppm)
YE elicitation time (days)
 0 730.7 ± 5.2d(E) 47.0 ± 3.1b(D) 3.47 ± 0.01e(J)
 5 796.0 ± 2.5c(D) 68.3 ± 1.3a(BC) 4.98 ± 0.01d(I)
 10 823.3 ± 3.5b(C) 70.0 ± 2.5a(ABC) 5.94 ± 0.01b(F)
 15 784.7 ± 9.8c(D) 72.7 ± 1.3a(ABC) 7.37 ± 0.01a(D)
 20 932.0 ± 0.6a(B) 74.0 ± 2.3a(AB) 5.60 ± 0.00c(G)
p values (one-way ANOVA) [YE, Elicitation time] 0.000*** 0.000*** 0.000***
MeJA elicitation time (days)
 0 302.0 ± 0.6d(G) 36.0 ± 0.0c(E) 5.50 ± 0.06e(H)
 5 543.0 ± 1.5c(F) 66.0 ± 2.1b(C) 13.94 ± 0.01b(B)
 10 732.0 ± 1.2b(E) 72.7 ± 2.9ab(ABC) 18.47 ± 0.02a(A)
 15 954.0 ± 2.3a(A) 76.3 ± 3.2a(A) 10.29 ± 0.01c(C)
 20 951.0 ± 2.6a(A) 73.0 ± 1.2ab(ABC) 7.11 ± 0.01b(E)
p values (one-way ANOVA) [MeJA, Elicitation time] 0.000*** 0.000*** 0.000***
Two-way ANOVA/general linear model
 Elicitor type (A) 0.000*** 0.266 ns 0.000***
 Elicitation time (B) 0.000*** 0.000*** 0.000***
 (A)*(B) 0.000*** 0.026* 0.000***
  1. Means (n = 30) ± standard error (S.E.) with the same letter in a column are not statistically significant different from each other according to the Duncan’s multiple range test at p ≤ 0.05. ns p > 0.05; *p ≤ 0.05; ***p ≤ 0.001. Superscript small letters—differences between five elicitation times for each elicitor type (either YE or MeJA) separately (one-way ANOVA). Superscript capital letters in parenthesis—differences between samples from the combined effect of two elicitor types and five elicitation times (two-way ANOVA)