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Table 4 Effect of different load amounts on Capto Butyl ImpRes

From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies

Capto Butyl ImpRes, pH 4.0 Load Monomer concentration (mg/mL) Monomer recovery (%) HCP (ppm) Purity (%)
HMW Mono LMW
FabscFv-KiH 20 mg/mL-R 0.93 81.1 235 0.8 98.3 0.9
40 mg/mL-R 1.34 90.8 243 1.3 98.0 0.7
75 mg/mL-R 1.54 94.4 1114 2.1 97.4 0.6
117 mg/mL-R 1.78 97.1 1713 2.7 96.4 0.9
Fab2scFv-KiH
(Post-Protein A eluate with pH 4.1 wash)
15 mg/mL-R 0.98 78.4 144 1.5 96.7 1.7
35 mg/mL-R 1.51 90.6 164 2.3 96.9 0.8
60 mg/mL-R 1.67 90.4 345 2.4 96.9 0.7