Table 4 Effect of different load amounts on Capto Butyl ImpRes
From: Effective flow-through polishing strategies for knob-into-hole bispecific antibodies
Capto Butyl ImpRes, pH 4.0 | Load | Monomer concentration (mg/mL) | Monomer recovery (%) | HCP (ppm) | Purity (%) | ||
|---|---|---|---|---|---|---|---|
HMW | Mono | LMW | |||||
FabscFv-KiH | 20Â mg/mL-R | 0.93 | 81.1 | 235 | 0.8 | 98.3 | 0.9 |
40Â mg/mL-R | 1.34 | 90.8 | 243 | 1.3 | 98.0 | 0.7 | |
75Â mg/mL-R | 1.54 | 94.4 | 1114 | 2.1 | 97.4 | 0.6 | |
117Â mg/mL-R | 1.78 | 97.1 | 1713 | 2.7 | 96.4 | 0.9 | |
Fab2scFv-KiH (Post-Protein A eluate with pH 4.1 wash) | 15Â mg/mL-R | 0.98 | 78.4 | 144 | 1.5 | 96.7 | 1.7 |
35Â mg/mL-R | 1.51 | 90.6 | 164 | 2.3 | 96.9 | 0.8 | |
60Â mg/mL-R | 1.67 | 90.4 | 345 | 2.4 | 96.9 | 0.7 | |