Fig. 4
From: A novel sustainable platform for scaled manufacturing of double-stranded RNA biopesticides
Effect of IPTG and induction time on dsRNA yield. AREV4 dsRNA construct was used in this experiment. Samples prepared in five replicates A t-test analysis comparing AREV4 dsRNA yield produced from 6-h induction in media A and E suggests no significance between the two media (t(8) = 9.149, p = 0.2490) with mean values for A and E = 11.50 and 18.42, respectively. B The relative yield of purified AREV4 dsRNA produced in media A and E expression systems was determined by measuring the main peak area (value of area indicated) chromatogram obtained by IP-RP-HPLC analysis. C t-test analysis of AREV4 dsRNA yield produced from 18-h induction in media A and E suggests no significance (t (8) = 0.9559, p = 0.6317) between the two media with mean values for A and E = 3.05 and 2.25, respectively. D t-test analysis of AREV4 dsRNA yield comparing 6-h induction vs 18-h induction using combined replicate samples (from media A and E) suggests there is a significant difference between the effects of length of induction times (t(18) = 9.630, p = 0.0042) with mean values for 6-induction and 18-h induction = 14.96 and 2.94, respectively