Skip to main content
Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Minos and Restless transposon insertion mutagenesis of psychrotrophic fungus for red pigment synthesis adaptive to normal temperature

Fig. 1

Strategy for the construction of transposable systems and screening of the positive mutants. Single system owns a single plasmid carrying transposase gene cassette and a known fragment with hygromycin resistance gene (hygR) and flanked by left (TIRL) and right (TIRR) terminal inverted repeats of transposons. Dual system consists of a donor plasmid with insertion fragment and a helper plasmid with transposase gene cassette. The transposase achieves transposition by excising the “known fragment” from the original plasmid and randomly integrating a new locus in host genome to select progeny changed in phenotype

Back to article page