High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris

Bioresources and Bioprocessing

Table 2 Amino acid analysis of hlCOLIII

Composition	Abbreviate	Assay (g/93 g)	Molar content (%)	Content (residues/1000 residues)
Aspartic acid	Asp (D)	8.26^a	7.69^a	77^a
Asparagine	Asn (N)	8.26^a	7.69^a	77^a
Glutamic acid	Glu (E)	20.82^b	17.60^b	176^b
Glutamine	Gln (Q)	20.82^b	17.60^b	176^b
Serine	Ser (S)	4.42	5.20	52
Histidine	His (H)	5.97	4.71	47
Glycine	Gly (G)	16.38	27.02	270
Threonine	Thr (T)	1.35	1.40	14
Arginine	Arg (R)	1.26	0.89	9
Alanine	Ala (A)	1.85	2.60	26
Tyrosine	Tyr (Y)	1.07	0.73	7
Cysteine	Cys (C)	0.00	0.00	0
Valine	Val (V)	1.68	1.77	18
Methionine	Met (M)	0.00	0.00	0
Phenylalanine	Phe (F)	1.48	1.11	11
Isoleucine	Ile (I)	0.00	0.00	0
Leucine	Leu (L)	0.00	0.00	0
Lysine	Lys (K)	6.30	5.33	53
Proline	Pro (P)	22.27	23.92	239

^{a, b}Asn and Gln will generate Asp and Glu, respectively, after acid hydrolysis treatment, so the values in the table are Asn + Asp and Gln + Glu