Table 1 Strains and plasmids used in this study
| Â | Characteristics | Source |
|---|---|---|
Strains | ||
E. coli DH5α E. coli BL21 | General cloning host General expressing host | The lab The lab |
E. coli ET12567 | Demethylated strain containing pUZ8002 plasmid for conjugative transfer with actinomycetes | The lab |
SF-2 | Streptomyces fradiae, neomycin-producing strains induced by ARTP | (Yu et al. 2022) |
BL21/pET28a | E. coli BL21 derivative harboring pET28a | The lab |
BL21/pET28a-NeoN | E. coli BL21 derivative harboring pET28a-NeoN | The lab |
BL21/pET28a-NeoNE34A | E. coli BL21 derivative harboring pET28a-NeoNE34A | This study |
BL21/pET28a-NeoNT38A | E. coli BL21 derivative harboring pET28a-NeoNT38A | This study |
BL21/pET28a-NeoND68A | E. coli BL21 derivative harboring pET28a-NeoND68A | This study |
BL21/pET28a-NeoNK36A | E. coli BL21 derivative harboring pET28a-NeoNK36A | This study |
BL21/pET28a-NeoNS251A | E. coli BL21 derivative harboring pET28a-NeoNS251A | This study |
BL21/pET28a-NeoNV252A | E. coli BL21 derivative harboring pET28a-NeoNV252A | This study |
ET12567/PPR-NeoN | E. coli ET12567 derivative harboring PPR-NeoN | This study |
ET12567/PPR-NeoNV252A | E. coli ET12567 derivative harboring PPR-NeoNV252A | This study |
ET12567/PKC1139-NeoN | E. coli ET12567 derivative harboring PKC1139-NeoN | This study |
SF-2-NeoN | SF-2 derivative the expression of NeoN | This study |
SF-2-NeoNV252A | SF-2 derivative the expression of NeoNV252A | This study |
SF-2ΔNeoN | SF-2 derivative the deletion of neoN | This study |
Plasmids | ||
pET28a | Kanr; Expression vector for the expression of target protein | The lab |
PPR (pSET152-PermE*) | E. coli–S. fradiae shuttle vector for the expression of target protein | The lab |
pKC1139 | E. coli–S. fradiae shuttle vector for the deletion of target protein | The lab |
pET28a-NeoN | Derived from pET28a, for induced expression of NeoN | This study |
pET28a-NeoNE34A | Derived from pET28a, for induced expression of NeoNE34A | Â |
pET28a-NeoNT38A | Derived from pET28a, for induced expression of NeoNT38A | This study |
pET28a-NeoND68A | Derived from pET28a, for induced expression of NeoND68A | This study |
pET28a-NeoNK36A | Derived from pET28a, for induced expression of NeoNK36A | This study |
pET28a-NeoNS251A | Derived from pET28a, for induced expression of NeoNS251A | This study |
pET28a-NeoNV252A | Derived from pET28a, for induced expression of NeoNV252A | This study |
PPR-NeoN | Derived from PPR, for the expression of NeoN | This study |
PPR-NeoNV252A | Derived from PPR, for the expression of NeoNV252A | This study |
PKC1139-ΔNeoN | Derived from PKC1139, for the deletion of neoN | This study |