Fig. 5From: Engineering a norcoclaurine synthase for one-step synthesis of (S)-1-aryl-tetrahydroisoquinolinesBiotransformation of benzaldehyde and dopamine catalysed by TfNCS and its mutants. The reaction mixture was composed of 10 mM amine, 1 mM aldehyde, 20% (v/v) DMSO, 5 mM ascorbic acid, HEPES buffer (100 mM, pH 7.5) and 0.5 mg mL−1 purified enzyme. Reactions were performed at 30 °C for 3 hBack to article page