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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extraction

Fig. 4

Lipid extraction yields and cell morphology with different methods. A Lipid extraction yields. Cells were treated according to different methods and then extracted with ethyl acetate at 30 ℃. B ESEM analysis of R. toruloides cells being treated with different methods. Method I. With no treatment; Method II. Heated in a boiling water bath for 20 min; Method III. Cells were separated, resuspended in water, heated in a boiling water bath for 20 min, separated and then resuspended in the supernatant of the corresponding culture broth; Method IV. Heated in a water bath at 50 ℃ for 24 h

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