Fig. 2
From: Efficient production of salvianic acid A from l-dihydroxyphenylalanine through a tri-enzyme cascade
Design and construct a tri-enzymatic cascade pathway to synthesize SAA from L-DOPA. a Designed SAA biosynthesis pathway by a tri-enzyme cascade pathway containing PmLAADM2, LaPPR and CbFDH; b effect of different activity ratios (0.1:1–3:1) of CbFDH/LaPPR on SAA production, with 15 g L−1 DHPPA, 16 g L−1 sodium formate in Tris–HCl buffer (50 mM, pH 7.0, containing 5 mM NAD+) in 30 ℃ for 12 h. c When the enzyme activity ratio of CbFDH: LaPPR was maintained at 0.3:1, effect of different activity ratios (0.2:1–3:1) of PmLAADM2/LaPPR on SAA production, with 15 g L−1 L-DOPA, 16 g L−1 sodium formate, in Tris–HCl buffer (50 mM, pH 7.0, containing 5 mM NAD+, 5 mM FAD) in 30 ℃ for 12 h. The data represent mean ± s.d., as determined from three independent experiments