Fig. 6
From: Efficient production of salvianic acid A from l-dihydroxyphenylalanine through a tri-enzyme cascade
One-pot production of SAA in vivo. a The gene organization in recombinant strains. Effect of copy number plasmids (b) and RBS (c) on SAA production with 50 g L−1 L-DOPA and 20 g L−1 wet cell in 10 mL Tris–HCl buffer (50 mM, pH 7.0, containing 3 mM NAD+ and 60 g L−1 sodium formate) in 30 °C for 12 h. d Effect of feed rate of L-DOPA on SAA production with 90 g L−1 L-DOPA (total) and 30 g L−1 wet cell in 100 mL Tris–HCl buffer (50 mM, pH 7.0, containing 0.6 mM NAD+ and 108 g L−1 sodium formate) in 30 °C for 12 h. The data represent mean ± s.d., as determined from three independent experiments. The substrate non-batch feeding mode was used as a contrast