Table 1 Kinetic parameters of the purified LaPPR WT and its mutants
From: Efficient production of salvianic acid A from l-dihydroxyphenylalanine through a tri-enzyme cascade
Mutants | Specific activity (U mg−1) | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) |
|---|---|---|---|---|
LaPPRWT | 5.8 ± 0.2 (1) | 6.20 ± 0.15 | 1.21 ± 0.03 | 0.20 (1) |
LaPPRH89M | 9.1 ± 0.3 (1.6) | 5.74 ± 0.08 | 1.37 ± 0.06 | 0.24 (1.2) |
LaPPR143D | 9.3 ± 0.3 (1.6) | 5.03 ± 0.14 | 2.08 ± 0.11 | 0.41 (2.1) |
LaPPRMu1 | 11.4 ± 0.3 (2.0) | 3.63 ± 0.17 | 2.35 ± 0.12 | 0.65 (3.3) |
LaPPRMu2 | 21.8 ± 0.7 (3.8) | 2.52 ± 0.27 | 5.74 ± 0.24 | 2.05 (10.3) |