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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Exploiting a heterologous construction of the 3-hydroxypropionic acid carbon fixation pathway with mesaconate as an indicator in Saccharomyces cerevisiae

Fig. 4

Optimization of MCR for the increased 3-HP accumulation. A Promoter optimization strategy for the expression of MCR. B 3-HP production in engineered yeasts from glucose by expression of MCR-C and MCR-N under control by different promoter combinations. C The competing synthetic pathway of 3-HP and fatty acid. Intracellular fatty acid synthesis competes with the 3-HP sub-pathway for catalyzing malonyl-CoA. D 3-HP production in engineered yeasts from glucose by expression of MCR using different plasmids, including 2 μ-based plasmid pSC-HIS-PH, 2 μ-based plasmid pSC-URA-PH, and ultrahigh copy number plasmid pUGG-PH. E The yield of the reporter mesaconate from glucose. M201, strain M2 harboring the plasmid pSC-HIS-MCR for overexpressing MCR; M203, strain M2 harboring the plasmid pUGG-PH for overexpressing MCR. Strains were cultured with SC-dropout medium with 0.1% glyoxylate

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